diploma in microbiology industrial training a.k.a PrAcTiCaL..!!

"In theory, theory and practice are the same. 

In practice, they are not." - Albert Einstein

Pada suatu hari, Liyana Yusof bertanyakan "hws practical life?..wat u do actually? tell me everything"

Alang2 rasa mcm kena cerita pnjg, so I'll just write it down here.

As we're aware, student semester akhir diploma mikrobiologi wajib menjalani latihan industri selama...plus minus, 3 bln..

Kat mana leh wat practical..?? Di mana2 saja yg ada kemudahan lab mikrobio/molecular..kilang Gardenia..MARDI..Agensi Nuklear (you'll be given a task/project..wat extract, experiment smua,,then present)..hospitals..institut bioteknologi and so on...kalau boleh, cari lah tmpt praktikal yg byk leh buat krja..kalau tak, nnti xtaw nk tulis apa dlm logbook n final report..

Disebabkan I've undergone my practical in hospital, so here, I'll share bout the practical experience in the hospital.  

Tanggal 2 Disember 2012, Ahad..the story begins~~

Hospital Sultanah Bahiyah

Memulakan perjalanan dgn kereta Porsche Kancil yg berusia 15thn..yg best praktikal kt Kedah..xyah risau2 about jln jam..so g lewat pun smpat smpai..hehehh..

Here's a thing..setiap hari ahad n rabu, kami ada kelas tutorial with the MLT (med lab tech) Chief..kelas ni..just refresh blk segala yg blajar n mcm mana nk apply dlm lab..

You're alone for the practical..?? don't worry, I was alone too..totally, I KNOW NO ONE THERE!!

What to do if you're alone??

• make friends!! first2 dtg tu salam2 la..Assalammualaikum..selamat pagi..student dr mana..?? this is my first day..tolong2 ajar n refreshkan bnda yg sy xtaw..can I join you for lunch..?? and smilessssss... :D  most of friends I've met there, still my friends even close friends until now..ngee...
• know the staffs!! Mostly staffs smua friendly but RESPECT them..tunjukkan gaya nk belajar. tunjukkan you have the knowledge in Microbiology field..first2 week tu biasala kena berleter bagai...mcm mana nk interpret biochemical tests..?? and i went... @___@   
• know the map!! first2 dtg Hosp. Sultanah Bahiyah...parking blakang forensics..pathology nun sebatu... =,=  hospital lain, I'm not quite sure..tp dkt HSBAS ni dlm paotologi ada byk lab..dan lab dlm lab dlm lab..selalu jgk tersesat~~
• UiTM!! bawalah nama universiti anda sebaiknya..org lain mgkin x ingat nama..but they'll call "student uitm yg itu yg ini"

Main point:: Buat apa time praktikal..?? Utk yg prktikal di hospitals, jadual anda akan dibahagikan ikut sections/rotations..the sections are based on human samples such as:

• serology/virology - test dengue, hepatitis, HIV, dan sgala yg brkaitan dgn virus..here, you'll get familiar with TORCHES, ASOT, ANA, Typhidot, Leptospira n so on...bnda apa tuh..?? don't worry, u'll learn..before that, wiki2 la dulu.. strong subject required: immunology.. yg pnting dlm seksyen ni u must understand the principle of antigen-antibody complex reaction. lab ni biasanya seminggu ja..but I got 2 weeks...rasa agak stress..!! sbb first week, nk adapt new environment..and coz I don't know what to do..!! lab serology ni agak relax sbb xbyk sgt krja...pipette this and that..masuk machine..dsebabkan virus-related..u can't really do everything..dan di sinilah..u'll realize..betapa sriyeznya ketenatan gejala sosial..on what grounds..?? HIV tests..16-years old girl, unmarried, and olredy has a baby boy..40-something teacher got HIV positive..this is the reality of our country..u got that everyday..
• bacteriology - blood, pus, urine, sputum, HVS, stool, TB, media (each sample, 1 week). Lab ni la yg sgt panjang n memenatkan..terutama kalau hospital tu adalah hospital besar..mcm HSBAS, we received samples from Klinik Kesihatan almost all over Kedah..Hospital Sg. Petani n Hospital Kangar jugak hntar smpel smpai ke sini..fuhhhh...HSBAS saja dkat 1000 patients. dh nama pun lab bacteriology..so strong subject, of course bacteriology n medical microbiology..

Bacteriology Lab

Bactec system

Bactec system ni nk test presence of bcteria in blood..msuk vial dlm machine..n ding..!! kalau ada bcteria, dia detect sndiri..type of pathogen..?? tu kna wat manual la...gram staining..kalau xtaw gak..u're not a microbiology student..huhu..(Staphylococcus in cluster, Streptococcus in chain) refresh blk the principle..acid-fast staining akan digunakan utk lab TB..

Basic things u MUST know are type of media..bcteria apa yg grow...here are the exmple...

• Blood agar - detect gram +; white colonies: Staphylococcus, lysis: Streptococcus, dark colonies: Enterococcus

Staphylococcus

Enterococcus

• Chocolate agar - detect Haemophilus Influenzae..kalo ada..smbung wat Satellitism n drug test..X+V smua tu..
• MacConkey agar - detect gram -; Lactose fermenter: pink colonies (E.coli, Klebsiella, Enterobacter), Non-lactose fermenter: white colonies

And other biochemical sets..Staphylococcus n Streptococcus, siapa coagulase positive..?? siapa catalase positive..?? gram negative, siapa yg motile..?? x-motile..?? bacteria mana produce H2S dlm TSI..?? hah..cari sndiri.. ;)

These are top common pathogens isolated:

• S. aureus
• E. coli
• Candida albicans
• Klebsiella pneumoniae
• Pseudomonas aeruginosa
• Streptococcus gp. B
• Acinetobacter baumanii
• Enterococcus faecium/faecalis
• Enterobacter aerogenes/clocae

*Enterobacter (gram negative), Enterococcus (gram positive)

Sebenarnya banyaaaaak bnda nk crita..don't worry..when u do it, u will see and u will understand..dlm second month u'll already get it..baca plates, biocemical test..less than 2 seconds, ding..!! E.coli..!! Kalau kat fakulti dulu,  kita hafal biochem set itu ini..so mmg xnmpak apa sbenarnya kita belajar..mcm mana nk identify bcteria..biasala..dlm lab main2 kann..time praktikal, dh nama pun praktikal..xyah hafal, u will understand..n u will see very crystal clear..dan sgt teratur..mcm mana nk tentukan biochemical set n so on...

Kalau dulu tngok human sample..blood, urine, stool..awrghhh...time prac..mcm main msk2~~ ada sekali wat microscopic exam (FEME) stool sample..atas slide mmg xnmpak apa2...tngok bwh microscope..... :O

ramaiiiii worms sdg menari2..

Anda bercita2 untuk menjadi doktor and any other health science related staffs...??? Berikut adalah pengajaran yg saya dapati stelah meng'observe' the real life of a hospital:

• Seriously doktor dh sgt ramaiii...mosttttly adalah housemen (HO)..beratus in this hospital only...ni yg dh grad...yg med student yg belum grad lg dh beribu...begitu juga dgn nurse n pharmacist...these courses are no longer critical courses in this country. FACT. Tp kalau anda betul2 ikhlas hati nk menceburi n nk berkhidmat demi jalanNya..in shaa Allah...Dia akan buka ruang dan pintu rezeki... :)
• Some young doctors..I have to say..rude...lalu lintas org mmg xtngok or tegur dh..kesian pakcik2 yg dok senyum meleret tnda respect..and..mmg mrupakan protokol..if u want to retrieve HIV test result, u must come to pathology urself ataupun hntar despatch to retrieve it for you..end up..MLT pulak yg kena marah2 knapa xleh gtaw melalui fon..nasib baik la MLT lab ni sabor orangnya..kalo den la haii..then bising2 xdpt result TB..sedangkan smple TB kena hntar BUKANNYA 3 kali berturut2..pathology has its own standard operating procedure..seriously..how do they get their medical liscence.. Respect others as you want to be respected.. even the MLTs coz they have much more experience in medical field than you are. 
• Doctors can threat a patient if there is a misdiagnose. Pathologist can threat a patient because we could lead to the misdiagnose..ngehehh..howw..?? yup, kalau salah label plate pun u could be in danger..why..?? with the wrong-labelled plate, we'd interpret the result wrongly and treat the healthy patient while we release the sick patient untreated. BEWARE! 

Cara nak lulus industrial training:

• bwk nama universiti elok2..tnjuk pnampilan yg baik..be punctual..!! respect the staffs..
• logbook..!! nk tulis apa..?? tulis jek everything you do in each day..kalau xtaw nk tulis apa..buat weekly..tulis la test apa..principle dia..mcm mana baca result..
• wat presentation kt pilah
• submit final report.... (mcm logbook jek..cuma dlm bntuk folio)

In a summary..first2 mmg rasa..useless nya daku..byk bnda xtaw..at the end..u'll realize u've learnt a lot..minggu2 terakhir..waa..terasa dipercayai bila staff tinggal jek smple suruh wat streaking n biochem tests..rasa cm kerja situ pulak..hehh...

habis2 praktikal..yess..!! lepas ni boleh bngun lambat..!! xyah iron baju..!! ;D